Within the framework of a study of the seismicity of the Aniene Valley (Central Italy), we analysed the medieval earthquakes of Subiaco (1216, 1227, 1299), the largest events reported for the area. Our main goal was to investigate some doubtful events reported in earthquake catalogues and, as such, currently utilised for seismic hazard estimates. A careful screening of the oldest available sources and their filiation pattern up to the present pointed out the uncertainty on the date and nature of these phenomena. A multidisciplinary approach based on the joint analysis of archaeological, geomorphologic and historical evidence allowed us to propose new interpretations concerning these events and their significance for the assessment of seismic hazard in the Aniene Valley. The main conclusion is that the dates of the 1216 and 1227 events are fairly unsupported. In particular, the 1216 earthquake could be dated back to between AD 1159 and 1181.